Regulatory

Part:BBa_K4907030

Designed by: Xinyi Yang   Group: iGEM23_XMU-China   (2023-09-13)


araBAD-weak

Usage and Biology

pBAD/araC promoter consists of two structures, namely araC and araBAD promoter. We found that BBa_K206001 and araBAD promoter part overlapped, so we tried to combine BBa_K206001 with the non-overlapping part to build an araBAD-weak promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 299
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


[edit]
Categories
Parameters
None